Bioanalysis Overview

XenoGesis offers state-of-the-art non-GLP bioanalysis

Whilst semi-quantitative analysis (i.e. without the use of an external calibration curve) allows the cost-effective comparison of individual samples, the use of matrix-matched calibration curves yields full quantification of unknown samples. For most drug discovery applications, our UHPLC-MS/MS services allow rapid, accurate and sensitive quantification from a wide variety of biological matrices.

Using ultra high performance liquid chromatography (UHPLC) on a range of different columns provides a significant improvement in resolution and throughput over conventional high performance liquid chromatography (HPLC) and combining this with tandem mass spectrometry (MS/MS) as the detector guarantees excellent specificity and sensitivity. This allows the bioanalysis of a wide range of compounds from very small polar molecules using hydrophilic interaction liquid chromatography (HILIC) to more conventional drug-like molecules and peptides.

We have recently expanded our LC/MS capabilities with the acquisition of a Thermo Scientific™ TSQ Quantiva™ Triple Quadrupole mass spectrometer and a Thermo Scientific™ Q Exactive™ Focus Hybrid Quadrupole-Orbitrap™. Both instruments have the Thermo Scientific™ Vanquish™ Flex Binary UHPLC systems which are state-of-the-art UHPLC systems allowing higher throughput, improved separations and robustness. The TSQ Quantiva is a modern, robust, highly sensitive triple quadrupole mass spectrometer which will be a valuable addition and complementary to our existing Waters TQ-S micro triple quadrupole mass spectrometer with an Acquity UHPLC system. The Q Exactive is a high resolution instrument providing accurate mass capability which will be invaluable for metabolite identification. Additionally, it offers an alternative to multiple reaction monitoring (MRM) by utilising the high resolution capability of the orbitrap either using high resolution full scan MS or MS/MS, or selected ion monitoring (SIM) for quantitation. This makes it ideal for screening and quantitation of compounds in numerous assays without the need for optimising compounds thereby saving time.

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